Characterization of a Diffusible Signaling Factor from Xylella fastidiosa

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Characterization of a Diffusible Signaling Factor from Xylella fastidiosa

UNLABELLED Cell-cell signaling in Xylella fastidiosa has been implicated in the coordination of traits enabling colonization in plant hosts as well as insect vectors. This cell density-dependent signaling has been attributed to a diffusible signaling factor (DSF) produced by the DSF synthase RpfF. DSF produced by related bacterial species are unsaturated fatty acids, but that of X. fastidiosa w...

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Promiscuous Diffusible Signal Factor Production and Responsiveness of the Xylella fastidiosa Rpf System

UNLABELLED Cell density-dependent regulation of gene expression in Xylella fastidiosa that is crucial to its switching between plant hosts and insect vectors is dependent on RpfF and its production of 2-enoic acids known as diffusible signal factor (DSF). We show that X. fastidiosa produces a particularly large variety of similar, relatively long-chain-length 2-enoic acids that are active in mo...

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Diffusible signal factor (DSF) synthase RpfF of Xylella fastidiosa is a multifunction protein also required for response to DSF.

Xylella fastidiosa, like related Xanthomonas species, employs an Rpf cell-cell communication system consisting of a diffusible signal factor (DSF) synthase, RpfF, and a DSF sensor, RpfC, to coordinate expression of virulence genes. While phenotypes of a ΔrpfF strain in Xanthomonas campestris could be complemented by its own DSF, the DSF produced by X. fastidiosa (XfDSF) did not restore expressi...

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Localization and characterization of Xylella fastidiosa haemagglutinin adhesins.

Xylella fastidiosa is a gram-negative, xylem-inhabiting, plant-pathogenic bacterium responsible for several important diseases including Pierce's disease (PD) of grapevines. The bacteria form biofilms in grapevine xylem that contribute to the occlusion of the xylem vessels. X. fastidiosa haemagglutinin (HA) proteins are large afimbrial adhesins that have been shown to be crucial for biofilm for...

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GENOMIC CHARACTERIZATION OF A LYSOGENIC PHAGE FROM XYLELLA FASTIDIOSA Principal Investigator:

Xylella fastidiosa (Xf) is an important pathogen causing diseases on several economically important crops, such as grape and almond, in California. Despite recent intensive efforts to study this nutritionally fastidious pathogen, many biological traits of the bacterium, such as bacteriophages, remain unclear and deserve more research attention. We have consistently observed the presence of phag...

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ژورنال

عنوان ژورنال: mBio

سال: 2013

ISSN: 2161-2129,2150-7511

DOI: 10.1128/mbio.00539-12